The variable regions database can also be populated with the output of other bioinformatics software, providing the flexibility to gather analyses from multiple sources. Variable chains identified through these analyses build up an internal database of variable regions and can be used to identify clonal populations from phage, hybridoma, and single B-cell campaigns. Out of the box, Affinity analysis pipelines can execute NCBI's IgBlast against a reference database of choice or perform a germline comparison against your custom database. Additional tools for searching Affinity's sequence database complement its analysis tools by rapidly honing in on sequences of interest and connecting them with information on associated proteins and plasmids. Spend time moving your discovery campaigns forward instead of getting multiple systems to cooperate in order to corral the data you need to make your decisions. The bioinformatics tools that perform these analyses must often either be licensed or built separately from an existing informatics system.Īnalysis of antibody sequences against germline or custom databases is built into Affinity's antibody discovery workflow, and Affinity's sequence analysis pipelines can be extended with your team's scripts. And whether your final goal is simply the sequence or recombinantly expressed purified antibody or molecular modeling of the CDRs, we have a solution that is right for you.Ĭontact us today to learn how we can advance your next drug discovery project.Antibody sequence analysis is an essential step in the antibody discovery process. So whatever your target, Antibody Solutions offers fast, reliable and high-quality sequencing services to advance your discovery along with a team of experts who can help you identify the best path forward. Confidentiality - All of the work we conduct takes place in our Santa Clara laboratory facility, sequence analyses are housed in a stand-alone onsite server rather than cloud-based options, and Antibody Solutions ensures that you retain full ownership of your IP.Flexibility - In addition to conventional rodents, we are the only CRO to generate antibodies and their related sequences from all four of the non-exclusive human antibody producing transgenic animal platforms available for therapeutic antibody discovery: Harbour Antibodies TM H2L2 mouse, Ligand’s OmniMouse ® and OmniRat ®, Trianni Mouse ® and Alloy Therapeutics’ ATX-GK ® mouse.Accuracy - We generate full-length, bidirectional Sanger sequence data directly from PCR products using germline-tailored primers and avoid unnecessary additional steps that could introduce error. Speed - As in “two-to-four weeks” in most cases, thanks in part to highly efficient methods for mRNA purification, cDNA generation and targeted PCR to obtain high throughput of samples.Variable region sequencing workflowĪnd just as we have for the tens of thousands of antibody clones we’ve generated since 1995, we apply the science and technical know-how we’ve mastered to deliver your sequences with: Bidirectional sequencing directly from PCR products eliminates the headaches and potential artifacts, error or bias that can be introduced by plasmid cloning and colony PCR.įigure 1. With years of technical knowledge and hands-on experience, we have successfully provided sequencing solutions for the full array of mouse and rat models we commonly utilize, including all of the human antibody-producing transgenic rodent models.Īntibody Solutions offers sample preparation and a comprehensive toolbox of reagents for sequencing of mouse, rat, human and transgenic immunoglobulin variable regions, in addition to full-length antibody sequencing services. Utilizing robust molecular biology techniques and Sanger sequencing methods, full-length, high-confidence reads of the entire antibody variable region are attainable. Whether you have a single lead clone or a panel of antibody candidates you want to sequence, with Antibody Solutions at your side, you can rapidly acquire your antibody sequences with high accuracy and low barriers to entry. Approaches to sequencing vary, and tough decisions need to be made on exactly when in the discovery timeline to obtain sequences - both cost and bandwidth play a role in that decision. Sequencing is a necessary step for scientists conducting research into therapeutic antibodies, CAR-T, bispecifics, T-cell engagers or other biologics. Sequencing solutions for conventional and transgenic animal models
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